Contamination of Fungus Cultures by Tyrophagus Lintneri12

Contamination of Tissue Cultures by Mycoplasmas

Mycoplasmas are the smallest and simplest self-replicating bacteria [1]. These microorganisms lack a peptidoglycan based rigid cell wall and thus are not susceptible to antibiotics, such as penicillin and its analogues, which are effective against most bacterial contaminants of cell cultures. The trivial name mycoplasma encompasses all species included in the class Mollicutes: i.e. the genera M...

Contamination of blood cultures.

detection of pestivirus contamination in cell cultures by nested-pcr

in this study a nested-pcr assay was optimized for detection of two bvdv biotype of nadl strain. a part of 5' non-coding region of virus, 249 bp in size, was amplified in rt-pcr. pcr product was cloned in a ptz57r/t vector and sequencing results confirmed the specificity of the test. internal primers were designed and a 155 bp dna fragment was amplified in nested-pcr. the sensitivity of rt...

Contamination of Blood Cultures 1055

Contamination of blood cultures is believed to occur mainly during the venepuncture procedure. Consequently, meticulous preparation of the venepuncture site is widely recommended. To determine whether the contamination rate is indeed affected by the quality of the antiseptic procedure at the venepuncture site, 181 paired cultures were collected from 176 patients during a 6-month period after ei...

Laboratory contamination of blood cultures.

A prospective study of the use of a laminar flow cabinet, an exhaust-ventilated safety hood, and the open bench for the microbiological examination of blood is described. Blood samples from 1600 patients were subcultured on the open bench, 2700 in a safety hood, and 2607 in a laminar flow cabinet. Use of the laminar flow cabinet produced a significantly greater level of contamination than the o...